human cardiomyocyte complete media with serum  (Celprogen Inc)

Journal: Frontiers in Toxicology

Article Title: Evaluation and investigation of the cardiotoxicity of the potential anti-cholangiocarcinoma drug lanatoside C

doi: 10.3389/ftox.2026.1783444

Figure Lengend Snippet: Impact of Lan C on cell viability of AC16 cells evaluated by CCK-8 assay. (A) Cell viability of AC16 cells treated with the indicated concentrations of Lan C for 12, 24, and 48 h. (B) Dose-response curve of cell inhibition on AC16 cell proliferation after 48-h treatment. The half maximal inhibitory concentration (IC50) is presented. **** P < 0.0001.

Article Snippet: Human AC16 cardiomyocytes (ATCC® CRL-3568TM) were employed for in vitro studies, with untreated cells included as negative controls.

Techniques: CCK-8 Assay, Inhibition, Concentration Assay

Journal: Frontiers in Toxicology

Article Title: Evaluation and investigation of the cardiotoxicity of the potential anti-cholangiocarcinoma drug lanatoside C

doi: 10.3389/ftox.2026.1783444

Figure Lengend Snippet: Effects of Lan C on Cell Viability and Reactive Oxygen Species (ROS) Levels in AC16 Cells. (A) Representative confocal microscopy images showing ROS-associated fluorescence intensity in cells treated with the indicated concentrations of Lan C for 48 h. (B) ROS fluorescence intensity was measured by flow cytometry in cells treated with the indicated concentrations of Lan C for 48 h. (C) Quantitative analysis of ROS fluorescence intensity obtained from flow cytometry. Data are presented as mean ± SD. ** P < 0.005, **** P < 0.0001. Scale bars: 100 μm.

Article Snippet: Human AC16 cardiomyocytes (ATCC® CRL-3568TM) were employed for in vitro studies, with untreated cells included as negative controls.

Techniques: Confocal Microscopy, Fluorescence, Flow Cytometry

Journal: Frontiers in Toxicology

Article Title: Evaluation and investigation of the cardiotoxicity of the potential anti-cholangiocarcinoma drug lanatoside C

doi: 10.3389/ftox.2026.1783444

Figure Lengend Snippet: Analysis of mitochondrial-mediated apoptotic pathway protein expression and lactate dehydrogenase (LDH) release in AC16 cells following Lan C treatment. (A) Representative Western blot bands showing protein expression levels of Bcl-2, cytochrome c, cleaved caspase-9, and cleaved caspase-3. (B–E) Quantitative analysis of the relative band intensity (gray value) for Bcl-2 (B) , cytochrome c (C) , cleaved caspase-9 (D) , and cleaved caspase-3 (E) . (F) The release rate of LDH into the extracellular medium after AC16 cells were treated with Lan C. * P < 0.05; ** P < 0.005; **** P < 0.0001.

Article Snippet: Human AC16 cardiomyocytes (ATCC® CRL-3568TM) were employed for in vitro studies, with untreated cells included as negative controls.

Techniques: Expressing, Western Blot